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Humanos , Feminino , Adulto , Staphylococcus , Tendões , Traumatismos da Mão , Abscesso , Tenossinovite , Infecções Estafilocócicas , Pacientes Internados , Exame Físico , Microbiologia , Técnicas MicrobiológicasAssuntos
Humanos , Masculino , Idoso , Antifúngicos , Hemocultura , Fusarium , Violeta Genciana , Coloração e Rotulagem , Fenazinas , OncologiaRESUMO
No disponible
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Humanos , Adulto , Endocardite , Kingella kingae , Ecocardiografia TransesofagianaAssuntos
Humanos , Feminino , Adulto , Antibacterianos/uso terapêutico , Celulite/tratamento farmacológico , Pacientes Internados , Exame Físico , Avaliação de Sintomas , Anamnese , Celulite/diagnóstico , Úlcera da Perna , Microbiologia , Doenças Transmissíveis , Dermatopatias , Resultado do TratamentoRESUMO
To document the epidemiology, clinical features, and outcomes of murine typhus patients in the Canary Islands (Spain), we analyzed data that were retrospectively collected for 16 years for 221 patients. Murine typhus in the Canary Islands is characterized by a high rate of complications (31.6%), mainly liver, lung, kidney or central nervous system involvement.
Assuntos
Tifo Endêmico Transmitido por Pulgas , Animais , Humanos , Fígado , Camundongos , Estudos Retrospectivos , Rickettsia typhi , Espanha/epidemiologia , Tifo Endêmico Transmitido por Pulgas/diagnóstico , Tifo Endêmico Transmitido por Pulgas/epidemiologiaRESUMO
No disponible
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Humanos , Masculino , Adulto , Obstrução Nasal/diagnóstico , Obstrução Nasal/microbiologia , Cavidade Nasal/diagnóstico por imagem , Obstrução Nasal/patologia , Biópsia , Cavidade Nasal/microbiologia , Bacilos e Cocos Aeróbios Gram-Negativos/isolamento & purificação , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Klebsiella pneumoniae/isolamento & purificaçãoRESUMO
No disponible
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Humanos , Masculino , Recém-Nascido , Conjuntivite Bacteriana/tratamento farmacológico , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Antibacterianos/uso terapêutico , Capnocytophaga , Conjuntivite Bacteriana/microbiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Testes de Sensibilidade Microbiana , RNA Bacteriano/análise , RNA Bacteriano/genéticaRESUMO
No disponible
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Humanos , Feminino , Adulto , Clostridium/isolamento & purificação , Infecções por Clostridium/diagnóstico , Insuficiência Renal Crônica/complicações , Peritonite/microbiologia , Clostridium/patogenicidade , Infecções por Clostridium/etiologia , Diálise Peritoneal/efeitos adversos , Testes de Sensibilidade MicrobianaRESUMO
La mayor parte de los casos de fiebre de duración intermedia (FDI) en España corresponden a enfermedades infecciosas (principalmente fiebre Q y rickettsiosis). En la práctica clínica el diagnóstico causal de estas entidades se basa en el inmunodiagnóstico, con una escasa utilidad en fases precoces. Por ello, el objetivo de este trabajo fue la evaluación de la utilidad de técnicas moleculares en el diagnóstico precoz de fiebre Q y rickettsiosis en pacientes con FDI. Se estudió mediante PCR la presencia de material genético de Coxiella burnetii y Rickettsia spp. en muestras sanguíneas de 271 pacientes con FDI. La especificidad de ambas técnicas es elevada, permitiendo el diagnóstico en casos no diagnosticados mediante detección de anticuerpos específicos. Estos datos sugieren que el empleo de técnicas moleculares, con una adecuada selección de la muestra de estudio y el empleo de cebadores adecuados, es un elemento útil en el diagnóstico precoz de las principales causas de FDI, principalmente si la serología es negativa o no es concluyente (AU)
Most cases of fever of intermediate duration (FDI) in Spain are associated with infectious diseases (mainly Q fever and rickettsia infections). In clinical practice, the causal diagnosis of these entities is based on immunodiagnostic techniques, which are of little help in the early stages. Therefore, the aim of this study was to evaluate the usefulness of molecular techniques for the early diagnosis of Q fever and rickettsia diseases in patients with FDI. A PCR method was used to detect the presence of genetic material of Coxiella burnetii and Rickettsia spp. in blood specimens from 271 patients with FDI. The specificity of both techniques is high, allowing diagnosis in cases undiagnosed by specific antibodies detection. These data suggest that the use of molecular techniques, with proper selection of the study specimen, and using appropriate primers is a useful tool in the early diagnosis of the main causes of FDI, especially if serology is negative or inconclusive (AU)
Assuntos
Humanos , Febre Q/diagnóstico , Febre Q/microbiologia , Diagnóstico Precoce , Infecções por Rickettsiaceae/microbiologia , Coxiella burnetii/isolamento & purificação , Infecções por Rickettsiaceae/complicações , Testes Imunológicos/métodos , Testes Sorológicos/métodosRESUMO
Coxiella burnetii, the etiological agent of human Q fever, can infect mammals, birds, and arthropods. The Canary Islands (Spain) are considered an endemic territory, with a high prevalence in both humans and livestock. Nonetheless, there is no epidemiological information about the wild and peridomestic cycles of C. burnetii. Tissue samples from rodents on farms (100) and wild rabbits (129) were collected and assessed by PCR to detect C. burnetii DNA. In parallel, ticks were also collected from vegetation (1169), livestock (335), domestic dogs (169), and wild animals (65). Globally, eight rodents (8%) and two rabbits (1.5%) were found to be positive, with the spleen being the most affected organ. Tick species identified were Hyalomma lusitanicum, Rhipicephalus turanicus, Rhipicephalus sanguineus, and Rhipicephalus pusillus. Hyalomma lusitanicum (80%) was the main species identified in vegetation, livestock, and wild animals, whereas Rhipicephalus sanguineus was the most prevalent in domestic dogs. Overall, C. burnetii DNA was detected in 6.1% of the processed ticks, distributed between those removed from livestock (11.3%), domestic dogs (6.9%), and from wild animals (6%). Ticks from vegetation were all negative. Results suggest that, in the Canary Islands, C. burnetii develops in a peridomestic rather than a wild cycle.
Assuntos
Animais Selvagens , Coxiella burnetii/isolamento & purificação , DNA Bacteriano/isolamento & purificação , Gado , Febre Q/veterinária , Animais , Doenças Endêmicas , Febre Q/epidemiologia , Espanha/epidemiologia , ZoonosesRESUMO
Most cases of fever of intermediate duration (FDI) in Spain are associated with infectious diseases (mainly Q fever and rickettsia infections). In clinical practice, the causal diagnosis of these entities is based on immunodiagnostic techniques, which are of little help in the early stages. Therefore, the aim of this study was to evaluate the usefulness of molecular techniques for the early diagnosis of Q fever and rickettsia diseases in patients with FDI. A PCR method was used to detect the presence of genetic material of Coxiella burnetii and Rickettsia spp. in blood specimens from 271 patients with FDI. The specificity of both techniques is high, allowing diagnosis in cases undiagnosed by specific antibodies detection. These data suggest that the use of molecular techniques, with proper selection of the study specimen, and using appropriate primers is a useful tool in the early diagnosis of the main causes of FDI, especially if serology is negative or inconclusive.
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DNA Bacteriano/sangue , Febre/etiologia , Técnicas de Diagnóstico Molecular , Reação em Cadeia da Polimerase/métodos , Febre Q/diagnóstico , Infecções por Rickettsia/diagnóstico , Bacteriemia/diagnóstico , Bacteriemia/microbiologia , Coxiella burnetii/genética , Coxiella burnetii/isolamento & purificação , Infecções por Citomegalovirus/diagnóstico , Diagnóstico Diferencial , Diagnóstico Precoce , Infecções por Vírus Epstein-Barr/diagnóstico , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Valor Preditivo dos Testes , Rickettsia/genética , Rickettsia/isolamento & purificação , Sensibilidade e Especificidade , Testes Sorológicos , Fatores de TempoRESUMO
INTRODUCTION: Shewanella spp. can cause severe skin and soft-tissue infections, gastrointestinal infections, otitis and bacteraemia, generally upon contact with seawater or consumption of raw seafood. Recently, a new condition termed 'patera foot' characterized by acute skin and soft-tissue infection has been described in irregular immigrants arriving to the Canary Islands, Spain, in rudimentary boats. Most infections are caused by a single species, Shewanella algae. The improvement of the diagnostic capabilities in clinical microbiology laboratories has resulted in a growing number of cases being reported worldwide, most of them coming from warm regions. CASE PRESENTATION: In this work, we reviewed the medical records of all the patients with Shewanella infections in the two university hospitals of Gran Canaria (the Canary Islands, Spain) during the period 2000-2016, resulting in the identification of 31 cases. We also conducted a literature review of Shewanella infections reported worldwide in recent years. CONCLUSION: This case series suggests that Shewanella infections are nosocomially acquired more frequently than previously thought. In addition, the unexpectedly high proportion of multidrug-resistant isolates raises concerns.
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